Brdu birthdating protocol
Brd U can be immunocytochemically detected in vitro and in vivo, allowing the identification of cells that were dividing the period of Brd U exposure.
In vivo, it has been used to identify the “birthdate” of cells during development, to examine the fate of postnatally generated cells, and to label cells before transplantation, for subsequent identification.
Adult neurogenesis is the lifelong generation of new neurons that occurs into restricted regions of the adult mammalian brain, namely the dentate gyrus of the hippocampus and the olfactory bulb.
In this chapter, we describe the procedures aimed to investigate adult neurogenesis in the murine brain.
Since the two chemicals are analogous, some spots of the genetic code that call for Thymidine will instead receive Brd U.
The special thing about Brd U is that it can be stained with an antibody using immunohistochemical methods, whereas Tritiated Thymidine is only detected by radiographic techniques.
(See a list of the products featured in this article.) Cell proliferation assays provide a critical piece of the puzzle when evaluating cell health, genotoxicity, and the efficacy of anti-cancer drugs.
Proliferation, however, is rarely assayed in isolation; other cell function probes are often used in concert with proliferation assays to provide a more informative picture of the state of the cell.
The Br atom acts as an anomalous scatterer and its larger size will affect the crystal's X-ray diffraction enough to detect isomorphous differences as well. A carbon substrate added to incubations of environmental samples will cause the growth of microorganisms that can utilize that substrate.Indirect methods such as those that measure mitochondrial activity (e.g.MTT) require additional confirmation since test agents could affect MTT processing without affecting cell viability.Brd U can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle during which DNA is replicated), substituting for thymidine during DNA replication.Antibodies specific for Brd U can then be used to detect the incorporated chemical (see immunohistochemistry), thus indicating cells that were actively replicating their DNA.
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Brd U is a synthetic analogue of Thymidine that is also incorportated into cells during DNA replication, which allows us to successfully identify dividing cells.